特色(se):
可用于檢測 R 環
對 DNA-RNA 雜交體的高特異性和親和力
不與單(dan)鏈 DNA 或(huo)雙鏈 DNA 發生交叉反應
對于富含 AU 的雙鏈 RNA,觀察到了輕微的交叉反應(ying)(約 5 倍以下)。
長度為 8、10、15 和 23 個堿基對(dui)的(de)雜(za)交體顯示出高(gao)親和力結合
DNA-RNA 雜(za)合(he)體是真核細胞中的一種(zhong)(zhong)自然(ran)現象(xiang),這些雜(za)合(he)體的水平在(zai)具有高轉(zhuan)錄活(huo)性的位點增加,例如在(zai)轉(zhuan)錄起始、抑(yi)制(zhi)和延(yan)伸期間。由(you)于(yu)(yu) RNA-DNA 雜(za)合(he)體會影響基(ji)因組(zu)的不穩定性,因此(ci) S9.6 抗(kang)體是一種(zhong)(zhong)有用的試劑,可幫助研究(jiu)在(zai) DNA 復制(zhi)或其他細胞過程中由(you)這些雜(za)合(he)體形成的 R 環和損傷的后(hou)果(guo)。此(ci)外,S9.6 抗(kang)體可有效識別用于(yu)(yu)微陣列(lie)研究(jiu)的 RNA-DNA 雜(za)交(jiao)。
This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.
Highlights:
* Useful in the detection of R-loops
* High specificity and affinity for DNA-RNA hybrids
* Does NOT cross-react with single-stranded DNA or double-stranded DNA
* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.
* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length
產品詳情:
Product Type: | Antibody |
Name: | Anti-DNA-RNA Hybrid [S9.6] |
Antigen: | S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen |
Isotype: | Rabbit IgG |
Fusion Tag(s): | Mouse Fab version contains His-tag |
Clone Name: | S9.6 |
Reactivity: | High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids |
Immunogen: | ΦX174 bacteriophage-derived synthetic DNA/RNA |
Purification Method: | Protein A/G |
Buffer: | ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300 |
Tested Applications: | Dot Blot Analysis: 0.2 µg/mL. |
參考文(wen)獻:
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